The expression and antigenicity of a truncated spike-nucleocapsid fusion protein of severe acute respiratory syndrome-associated coronavirus
Identifieur interne : 002F91 ( Main/Exploration ); précédent : 002F90; suivant : 002F92The expression and antigenicity of a truncated spike-nucleocapsid fusion protein of severe acute respiratory syndrome-associated coronavirus
Auteurs : Feng Mu [République populaire de Chine] ; Dongsheng Niu [République populaire de Chine] ; Jingsong Mu [République populaire de Chine] ; Bo He [République populaire de Chine] ; Weiguo Han [République populaire de Chine] ; Baoxing Fan [République populaire de Chine] ; Shengyong Huang [République populaire de Chine] ; Yan Qiu [République populaire de Chine] ; Bo You [République populaire de Chine] ; Weijun Chen [République populaire de Chine]Source :
- BMC Microbiology [ 1471-2180 ] ; 2008.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Antigènes viraux (immunologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Humains, Immunité cellulaire, Production d'anticorps, Prolifération cellulaire (), Protéines de fusion virale (génétique), Protéines de fusion virale (immunologie), Protéines de fusion virale (pharmacologie), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Rate (cytologie), Régulation de l'expression des gènes viraux, Réplication virale, Sensibilité et spécificité, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (), Test ELISA, Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (physiologie).
- MESH :
- cytologie : Rate.
- génétique : Glycoprotéines membranaires, Protéines de fusion virale, Protéines de l'enveloppe virale, Protéines nucléocapside, Virus du SRAS.
- immunologie : Anticorps antiviraux, Antigènes viraux, Glycoprotéines membranaires, Protéines de fusion virale, Protéines de l'enveloppe virale, Protéines nucléocapside, Virus du SRAS.
- pharmacologie : Protéines de fusion virale.
- physiologie : Virus du SRAS.
- Animaux, Glycoprotéine de spicule des coronavirus, Humains, Immunité cellulaire, Production d'anticorps, Prolifération cellulaire, Régulation de l'expression des gènes viraux, Réplication virale, Sensibilité et spécificité, Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère, Test ELISA.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (immunology), Antibody Formation, Antigens, Viral (immunology), Cell Proliferation (drug effects), Enzyme-Linked Immunosorbent Assay, Gene Expression Regulation, Viral, Humans, Immunity, Cellular, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (physiology), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (prevention & control), Spike Glycoprotein, Coronavirus, Spleen (cytology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Fusion Proteins (genetics), Viral Fusion Proteins (immunology), Viral Fusion Proteins (pharmacology), Virus Replication.
- MESH :
- chemical , genetics : Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins, Viral Fusion Proteins.
- chemical , immunology : Antibodies, Viral, Antigens, Viral, Membrane Glycoproteins, Nucleocapsid Proteins, Viral Envelope Proteins, Viral Fusion Proteins.
- cytology : Spleen.
- drug effects : Cell Proliferation.
- genetics : SARS Virus.
- immunology : SARS Virus.
- chemical , pharmacology : Viral Fusion Proteins.
- physiology : SARS Virus.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Antibody Formation, Enzyme-Linked Immunosorbent Assay, Gene Expression Regulation, Viral, Humans, Immunity, Cellular, Mice, Mice, Inbred BALB C, Sensitivity and Specificity, Spike Glycoprotein, Coronavirus, Virus Replication.
Abstract
In the absence of effective drugs, controlling SARS relies on the rapid identification of cases and appropriate management of the close contacts, or effective vaccines for SARS. Therefore, developing specific and sensitive laboratory tests for SARS as well as effective vaccines are necessary for national authorities.
Genes encoding truncated nucleocapsid (N) and spike (S) proteins of
The truncated S-N fusion protein is a suitable immunodiagnostic antigen and vaccine candidate.
The online version of this article (doi:10.1186/1471-2180-8-207) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1471-2180-8-207
PubMed: 19038059
PubMed Central: 2613400
Affiliations:
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Le document en format XML
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(génétique)</term><term>Glycoprotéines membranaires (immunologie)</term><term>Humains</term><term>Immunité cellulaire</term><term>Production d'anticorps</term><term>Prolifération cellulaire ()</term><term>Protéines de fusion virale (génétique)</term><term>Protéines de fusion virale (immunologie)</term><term>Protéines de fusion virale (pharmacologie)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Rate (cytologie)</term><term>Régulation de l'expression des gènes viraux</term><term>Réplication virale</term><term>Sensibilité et spécificité</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère ()</term><term>Test ELISA</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antibodies, Viral</term><term>Antigens, Viral</term><term>Membrane Glycoproteins</term><term>Nucleocapsid Proteins</term><term>Viral Envelope Proteins</term><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Rate</term></keywords><keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Spleen</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Proliferation</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps antiviraux</term><term>Antigènes viraux</term><term>Glycoprotéines membranaires</term><term>Protéines de fusion virale</term><term>Protéines de l'enveloppe virale</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Protéines de fusion virale</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Viral Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antibody Formation</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Gene Expression Regulation, Viral</term><term>Humans</term><term>Immunity, Cellular</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Sensitivity and Specificity</term><term>Spike Glycoprotein, Coronavirus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Immunité cellulaire</term><term>Production d'anticorps</term><term>Prolifération cellulaire</term><term>Régulation de l'expression des gènes viraux</term><term>Réplication virale</term><term>Sensibilité et spécificité</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>In the absence of effective drugs, controlling SARS relies on the rapid identification of cases and appropriate management of the close contacts, or effective vaccines for SARS. Therefore, developing specific and sensitive laboratory tests for SARS as well as effective vaccines are necessary for national authorities.</p></sec><sec><title>Results</title><p>Genes encoding truncated nucleocapsid (N) and spike (S) proteins of <italic>SARSCoV</italic> were cloned into the expression vector <italic>pQE30</italic> and fusionally expressed in <italic>Escherichia coli</italic> M15. The fusion protein was analyzed for reactivity with SARS patients' sera and with anti-sera against the two human coronaviruses <italic>HCoV</italic> 229E and <italic>HCoV</italic> OC43 by ELISA, IFA and immunoblot assays. Furthermore, to evaluate the antigen-specific humoral antibody and T-cell responses in mice, the fusion protein was injected into 6-week-old BALB/c mice and a neutralization test as well as a T-cell analysis was performed. To evaluate the antiviral efficacy of immunization, BALB/c mice were challenged intranasally with <italic>SARSCoV</italic> at day 33 post injection and viral loads were determined by fluorescent quantitative RT-PCR. Serological results showed that the diagnostic sensitivity and specificity of the truncated S-N fusion protein derived the SARS virus were > 99% (457/460) and 100.00% (650/650), respectively. Furthermore there was no cross-reactivity with other two human coronaviruses. High titers of antibodies to <italic>SRASCoV</italic> appeared in the immunized mice and the neutralization test showed that antibodies to the fusion protein could inhibit <italic>SARSCoV</italic>. The T cell proliferation showed that the fusion protein could induce an antigen-specific T-cell response. Fluorescent quantitative RT-PCR showed that BALB/c mice challenged intranasally with <italic>SARSCoV</italic> at day 33 post injection were completely protected from virus replication.</p></sec><sec><title>Conclusion</title><p>The truncated S-N fusion protein is a suitable immunodiagnostic antigen and vaccine candidate.</p></sec><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1471-2180-8-207) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Marra, Ma" uniqKey="Marra M">MA Marra</name></author><author><name sortKey="Jones, Sjm" uniqKey="Jones S">SJM Jones</name></author><author><name sortKey="Astell, Cr" uniqKey="Astell C">CR Astell</name></author><author><name sortKey="Holt, Ra" uniqKey="Holt R">RA Holt</name></author><author><name sortKey="Wilson, 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Chine"><noRegion><name sortKey="Mu, Feng" sort="Mu, Feng" uniqKey="Mu F" first="Feng" last="Mu">Feng Mu</name></noRegion><name sortKey="Chen, Weijun" sort="Chen, Weijun" uniqKey="Chen W" first="Weijun" last="Chen">Weijun Chen</name><name sortKey="Fan, Baoxing" sort="Fan, Baoxing" uniqKey="Fan B" first="Baoxing" last="Fan">Baoxing Fan</name><name sortKey="Han, Weiguo" sort="Han, Weiguo" uniqKey="Han W" first="Weiguo" last="Han">Weiguo Han</name><name sortKey="He, Bo" sort="He, Bo" uniqKey="He B" first="Bo" last="He">Bo He</name><name sortKey="Huang, Shengyong" sort="Huang, Shengyong" uniqKey="Huang S" first="Shengyong" last="Huang">Shengyong Huang</name><name sortKey="Mu, Jingsong" sort="Mu, Jingsong" uniqKey="Mu J" first="Jingsong" last="Mu">Jingsong Mu</name><name sortKey="Niu, Dongsheng" sort="Niu, Dongsheng" uniqKey="Niu D" first="Dongsheng" last="Niu">Dongsheng Niu</name><name sortKey="Qiu, Yan" sort="Qiu, Yan" uniqKey="Qiu Y" first="Yan" last="Qiu">Yan Qiu</name><name sortKey="You, Bo" sort="You, Bo" uniqKey="You B" first="Bo" last="You">Bo You</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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